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D9542

Sigma-Aldrich

DAPI

for nucleic acid staining

别名:

4′,6-二脒基-2-苯基吲哚 二盐酸盐, 2-（4-氨基苯基）-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐

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About This Item

经验公式（希尔记法）:
C16H15N5 · 2HCl
CAS号:
28718-90-3
分子量:
350.25
Beilstein:
4894417
EC號碼:
249-186-7
MDL號碼:
MFCD00012681
分類程式碼代碼:
12352200
PubChem物質ID:
24894305
NACRES:
NA.52

等級

Molecular Biology
for molecular biology

化驗

≥98% (HPLC)

形狀

powder

技術

transfection: suitable

溶解度

H2O: 20 mg/mL
PBS: insoluble

&epsilon ；(消光係數)

30 at 263 nm in H2O at 1 mM

螢光

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI 密鑰

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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相关类别

一般說明

DAPI（4′,6-二脒基-2-苯基吲哚二盐酸盐）是一种可与DNA结合的细胞可通透荧光染料。
heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.

應用

在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。
在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱，通过对双链复合体的特殊可视化检测印迹应用中的退火探针，并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。
适用于
  • 琼脂糖凝胶中的DNA染色
  • 分析凋亡过程中DNA的变化
  • 支原体检测
  • DNA的光足迹图谱
  • 细胞的免疫荧光染色

DAPI已用于：
  • 微生物污染的快速监测
  • 染色体条带技术
  • 凋亡细胞的检测
  • 在荧光显微镜中追踪DisA（DNA完整性扫描蛋白）在枯草芽孢杆菌DNA上的移动
  • 对成熟的花粉粒进行染色（0.5 mg/ml）

生化／生理作用

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟（富含AT的DNA优先）结合，形成稳定的复合物，其发出的荧光比单独的DAPI高约20倍。

注意

避光保存。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

相關產品

产品编号
说明
价格

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H315,H317,H335

危險分類

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
098M4004V
13190309
079M4003V
059M4025V
13190123

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Yona Goldshmit et al.
Brain and behavior, 4(2), 187-200 (2014-04-01)
A major impediment for recovery after mammalian spinal cord injury (SCI) is the glial scar formed by proliferating reactive astrocytes. Finding factors that may reduce glial scarring, increase neuronal survival, and promote neurite outgrowth are of major importance for improving
K Nakajima et al.
Letters in applied microbiology, 40(2), 128-132 (2005-01-13)
To apply fluorescent staining method for fast assessment of microbial quality of herbal medicines. The number of total bacteria and esterase-active bacteria on powdered traditional Chinese medicines were enumerated by fluorescent staining method using 6-carboxyfluorescein diacetate (6CFDA) and 4',6-diamidino-2-phenylindole (DAPI)
Yan Li et al.
Toxicology letters, 154(3), 225-233 (2004-10-27)
Excessive exposure to synthetic and endogenous estrogens has been associated with the development of cancer in several tissues including the breast. 4-Hydroxyequilenin (4-OHEN), a major catechol metabolite of equine estrogens present in Premarin, an estrogen replacement formulation, has been shown
Michal Bejerano-Sagie et al.
Cell, 125(4), 679-690 (2006-05-23)
In response to DNA damage, cells activate checkpoint signaling cascades to control cell-cycle progression and elicit DNA repair in order to maintain genomic integrity. The sensing and repair of lesions is critical for Bacillus subtilis cells entering the developmental process
R Howden et al.
Genetics, 149(2), 621-631 (1998-06-11)
As a strategy for the identification of T-DNA-tagged gametophytic mutants, we have used T-DNA insertional mutagenesis based on screening for distorted segregation ratios by antibiotic selection. Screening of approximately 1000 transgenic Arabidopsis families led to the isolation of eight lines
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