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268298

Sigma-Aldrich

DAPI,二盐酸盐

Cell-permeable DNA-binding dye.

别名:

DAPI,二盐酸盐, 4ʹ,6-二脒基-2-苯基吲哚二盐酸盐

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About This Item

经验公式(希尔记法):
C16H15N5 · 2HCl
CAS号:
分子量:
350.25
MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.54

品質等級

化驗

≥90% (HPLC)

形狀

solid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
desiccated (hygroscopic)
protect from light

顏色

yellow

溶解度

water: 5 mg/mL
ethanol: soluble
methanol: soluble

螢光

λex ~359 nm
λem ~461 nm

運輸包裝

ambient

儲存溫度

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI 密鑰

FPNZBYLXNYPRLR-UHFFFAOYSA-N

一般說明

DNA特异性荧光染料。优先结合富含腺嘌呤和胸苷的DNA(最大激发=359 nm,最大发射=461 nm)。可用于凋亡细胞中细胞核和核DNA的显微镜分析。可用于检测支原体。S-腺苷-L-蛋氨酸脱羧酶和二胺氧化酶的可逆抑制剂。细胞通常以1 µg/ml染色。
细胞可渗透的DNA-结合染料。优先与富含腺嘌呤和胸腺嘧啶的DNA结合。在流式细胞仪中用于测量核DNA含量或对分离的染色体进行分选。可用于正常和凋亡细胞中细胞核和核DNA的显微镜检测。可用于检测支原体。S-腺苷-L-蛋氨酸脱羧酶和二胺氧化酶的可逆抑制剂。也可以5 mg/ml mM的H2O溶液(目录号508741)提供。

生化/生理作用

主要靶标
优先与富含腺嘌呤和胸腺嘧啶的DNA结合
产物不与ATP竞争。
可逆性:是
细胞渗透性:否

警告

毒性:标准处理(A)

重構

复溶后,等分并冷冻长期保存(-20°C),或冷藏(4°C)短期保存。储备溶液在4°C下可稳定保存6周,在-20°C下可稳定保存6个月。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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