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345811

Sigma-Aldrich

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli, hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures.

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About This Item

EC 号:
3.2.1.103 (BRENDA, IUBMB)
分類程式碼代碼:
12352202
NACRES:
NA.54

重組細胞

expressed in E. coli

品質等級

100

共軛

(Glucosaminoglycan)

形狀

liquid

比活性

≥14 U/mL
≥140 units/mg protein

製造商／商標名

Calbiochem®

儲存條件

OK to freeze

運輸包裝

ambient

儲存溫度

2-8°C

相关类别

一般說明

Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.

外觀

Sterile-filtered in 20 mM Tris-HCl, pH 7.5.

重構

There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.

其他說明

Murata, T., et al. 2003. Eur. J. Biochem.270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.
Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.
Scudder, P., et al. 1983. Biochem. J.213, 485.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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