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Merck

G6008

Sigma-Aldrich

β 半乳糖苷酶 来源于大肠杆菌

Grade VI, lyophilized powder, ≥250 units/mg protein

别名:

β-D-半乳糖苷半乳糖水解酶, 乳糖酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Escherichia coli

品質等級

種類

Grade VI

形狀

lyophilized powder

比活性

≥250 units/mg protein

分子量

465 kDa

不包含

BSA as extender

成份

Protein, ≥50% biuret

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

β-半乳糖苷酶是由四个相同亚基组成的四聚体,每个亚基为135,000 Da。它是一种含巯基的酶,每个亚基含有19个半胱氨酸残基。

應用

β-半乳糖苷酶与特异性识别目标分子的抗体偶联(酶联免疫检测或 EIA)。 β-半乳糖苷酶还被用作监测启动子基因表达水平的报告酶。

生化/生理作用

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
β-半乳糖苷酶将乳糖切割成单糖成分,即葡萄糖和半乳糖。它还在反馈环中催化葡萄糖的转糖基化,生成β-半乳糖苷酶的诱导物——异乳糖

物理性質

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

單位定義

在 37 °C,pH 7.3 条件下,一个单位每分钟可将 1.0μmole 邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和 D-半乳糖。

外觀

部分纯化;含有Tris缓冲盐、氯化镁、DL-二硫苏糖醇和 2-巯基乙醇

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
G R CRAVEN et al.
The Journal of biological chemistry, 240, 2468-2477 (1965-06-01)
D C Young et al.
Analytical biochemistry, 215(1), 24-30 (1993-11-15)
Bacterial beta-galactosidase is one of several reporter enzymes used in studying the transcriptional activity of eukaryotic promoters. Although it is one of the easiest and least expensive enzymes to assay, its use has been limited because of its low sensitivity
K Kato et al.
Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
D H Juers et al.
Protein science : a publication of the Protein Society, 8(1), 122-136 (1999-04-21)
Beta-galactosidase (lacZ) from Escherichia coli is a 464 kDa homotetramer. Each subunit consists of five domains, the third being an alpha/beta barrel that contains most of the active site residues. A comparison is made between each of the domains and

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