219373

Cathepsin G, Human Neutrophil

Cathepsin G, Human Neutrophil, CAS 107200-92-0, is a purified native cathepsin G. Acts as a potent agonist of human platelet activation leading to their aggregation.

• 别名: Cathepsin G, Human Neutrophil
• CAS号: 107200-92-0
• EC 号: 3.4.21.20 (BRENDA, IUBMB)
• MDL號碼: MFCD00130750
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 生物源: human neutrophils
• 品質等級: 100
• 描述: Merck USA index - 14, 1905
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized solid (Salt-free)
• 比活性: ≥2 units/mg protein
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 技術: inhibition assay: suitable
• 適合性: suitable for molecular biology
• 應用: life science and biopharma
• 運輸包裝: ambient
• 儲存溫度: −20°C
• 基因資訊: human ... CTSG(1511)

说明

一般說明

Research area: Cell Signaling

Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.

應用

Cathepsin G, Human Neutrophil has been used to determine the in vitro bactericidal activities of cathepsin G and for in vitro digestion of plasma/acute wound fluids.

生化／生理作用

This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8. Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux. Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-AAPF-pNA per min at 25°C, pH 7.5.

準備報告

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.

重構

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -70°C.

Reconstitute in 50 mM NaOAc and 150 mM NaCl, pH 5.5.

其他說明

Glusa, E., and Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable