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一般說明
弹性蛋白酶是 240 个氨基酸残基的单一多肽链，含有四个二硫键。分子量约为 25.9kDa。该酶被合成为无活性的酶原，原弹性蛋白酶，其通过胰蛋白酶在 N-末端的有限蛋白水解而转化为活性形式。
應用
来自猪胰腺的弹性蛋白酶用于以下应用：
- 细胞培养技术
- ELISA 技术
- 用于测定弹性蛋白酶抑制活性
生化／生理作用
弹性蛋白酶水解弹性蛋白（弹性纤维的特定蛋白质）并消化血红蛋白、酪蛋白和纤维蛋白。
降低弹性蛋白的酶，弹性蛋白是参与拉伸过程的组织的基质成分。它是一种具有广泛特异性的丝氨酸蛋白酶。它切割小疏水性氨基酸（如 Ile、Gly、Ala、Ser、Val 和 Leu）的羧基侧的蛋白质。该酶还水解酰胺和酯，例如 N-苯甲酰基-L-丙氨酸甲酯。
包裝
包装尺寸基于蛋白质含量
單位定義
一个单元将在 25℃ 下，每分钟，pH8.0，水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。
基底
應用
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
P Hamet et al.
The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed by its egression into plasma and urine. Since the extracellular appearance of this cyclic nucleotide is used as
F Seibold et al.
Gut, 32(10), 1192-1197 (1991-10-01)
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The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid
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实验方案
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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