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Key Documents

16-201

Sigma-Aldrich

蛋白质A琼脂糖/鲑鱼精子DNA,2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

别名:

ChIP agarose beads, ChIP agaraose G beads

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.32

品質等級

形狀

liquid

製造商/商標名

Upstate®

技術

ChIP: suitable

適合性

suitable for immunoprecipitation

運輸包裝

wet ice

一般說明

重组蛋白G对IgG分子的Fc区具有非常高的亲和力。将蛋白G固定在微珠上会形成一种亲和树脂,可用于从粗血清、腹水或细胞培养基中纯化IgG组分。
重组蛋白G通过溴化氰键与琼脂糖共价结合。当收集用于染色质免疫沉淀(ChIP分析)的免疫复合物时,超声处理的鲑鱼精子DNA会阻断蛋白G琼脂糖上的非特异性DNA结合位点。

包裝

2.5 mL填充珠以50%凝胶浆形式提供,最终体积为5 mL。

品質

在染色质免疫沉淀中常规评估,用于预先清除丁酸钠处理的HeLa细胞的上清液,以及随后与乙酰化组蛋白交联的DNA的免疫沉淀

外觀

含有1 mg超声鲑鱼精子DNA、2.5 mg BSA和约5 mg重组蛋白G的2.5 mL填充珠,以50%凝胶浆形式提供,每瓶最终体积为5 mL。 混悬在含有0.05%叠氮化钠的10 mM Tris-HCl,1 mM EDTA(pH 8.0)中。 液体混悬液。

儲存和穩定性

自发货之日起在4°C下可保存1年。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


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