推荐产品
生物源
human
品質等級
分子量
Mw 21 kDa
製造商／商標名
Upstate®
濃度
>50% (Coomassie blue staining, SDS-PAGE)
2 mg/mL
技術
ELISA: suitable
affinity binding assay: suitable (G-protein)
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
一般說明
产品来源：GST融合蛋白，对应于在大肠埃希菌中表达的人PAK-1的p21结合结构域（PBD，残基67-150）。
品質
通过G-蛋白活性测定法进行常规评估
外觀
300 µg PAK-1 PBD，GST，溶于150 µL PBS（pH 7.4），0.5 mM PMSF（包含20%甘油）中。
儲存和穩定性
自发运之日起，在-20℃下可稳定保存1年。 为了最大程度地回收产品，在取下盖子之前，将原始样品瓶进行离心。
分析報告
比活度：亲和沉淀测定： 当加入10 µL谷胱甘肽琼脂糖时，10 µg该批次从体外加载GTPγS的3T3和A431细胞裂解物中沉淀出Rac-GTP。 经3T3和A431裂解物处理的GDP用作阴性对照。 在蛋白质印迹法中，使用1 &μg/mL抗Rac（目录号05-389）和山羊抗小鼠HRP二抗（目录号12-349）通过免疫印迹分析检测沉淀的Rac-GTP。
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
A Ros-Baro et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(21), 12050-12055 (2001-10-11)
It has been recently reported that insulin recruits a novel signaling machinery to lipid rafts required for insulin-stimulated GLUT4 translocation [Baumann, A., Ribon, V., Kanzaki, M., Thurmond, D. C., Mora, S., Shigematsu, S., Bickel, P. E., Pessin, J. E. &
Shamsideen A Ojelade et al.
PloS one, 10(9), e0137465-e0137465 (2015-09-15)
Responses to the effects of ethanol are highly conserved across organisms, with reduced responses to the sedating effects of ethanol being predictive of increased risk for human alcohol dependence. Previously, we described that regulators of actin dynamics, such as the
V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although
Yuna Guo et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(22), 5064-5072 (2015-06-14)
We previously identified the R-enantiomer of ketorolac as an inhibitor of the Rho-family GTPases Rac1 and Cdc42. Rac1 and Cdc42 regulate cancer-relevant functions, including cytoskeleton remodeling necessary for tumor cell adhesion and migration. This study investigated whether administration of racemic
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门