推荐产品
品質等級
無菌
sterile-filtered
形狀
liquid
儲存條件
protect from light
技術
cell culture | embryo: suitable
雜質
endotoxin, tested
成分
HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
運輸包裝
ambient
儲存溫度
2-8°C
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應用
推荐用于室温下操作小鼠胚胎。M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的Krebs-Ringer碳酸氢盐溶液，与Whitten′s培养基非常相似。M16含有丙酮酸和乳酸作为能量来源，因为植入前胚胎不能有效利用葡萄糖。
M2培养基是对M16的进一步改良，用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。
M2培养基是对M16的进一步改良，用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。
品質
溶液中的沉淀不影响本品性能。
重構
添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。
分析報告
该产品经过测试，能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80％。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Jane C Fenelon et al.
Biology of reproduction, 97(1), 119-132 (2017-06-24)
Embryonic diapause is a common reproductive strategy amongst mammals, requiring an intimate cross-talk between the endometrium and the blastocyst. To date, the precise molecular signals responsible are unknown in the mouse or any other mammal. Previous studies in the mink
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Yixin Xu et al.
Gene expression patterns : GEP, 25-26, 46-58 (2017-06-07)
Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic
Sarah E M Stephenson et al.
Scientific reports, 8(1), 7528-7528 (2018-05-16)
Mutations in PARK2 (parkin) can result in Parkinson's disease (PD). Parkin shares a bidirectional promoter with parkin coregulated gene (PACRG) and the transcriptional start sites are separated by only ~200 bp. Bidirectionally regulated genes have been shown to function in common
实验方案
利用 CRISPR-Cas9 基因组编辑技术创建转基因小鼠
Creating Transgenic Mice using CRISPR-Cas9 Genome Editing
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