05-389-AF488

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor^™ 488 Conjugate

clone 23A8, from mouse, ALEXA FLUOR^™ 488

• 别名: Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, Rac1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 23A8, monoclonal
• 物種活性: rat, mouse, human
• 技術: immunocytochemistry: suitable
• 同型: IgG2b
• NCBI登錄號: NM_006908
• UniProt登錄號: P63000
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RAC1(5879)

说明

一般說明

Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions, including cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family consists of 22 members, which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamily (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.

特異性

Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.

免疫原

Recombinant protein containing the full length human Rac.

應用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rac1 in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Rac1 Antibody, clone 23A8, Alexa Fluor^™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of Rac1.

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in A431 cells.

標靶描述

~21 kDa observed

外觀

Protein G Purified

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable