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一般說明
用于全转录组扩增(WTA)的SeqPlex™RNA扩增试剂盒(SEQXE)专用于对少量或降解/高度片段化的RNA（如来自福尔马林固定石蜡包埋（FFPE）组织样本的RNA ）进行下一代测序(NGS)。通过SeqPlex试剂盒可对这类RNA以及其它用于NGS的少量/高度片段化RDNA样品进行预扩增。它还有助于扩增从组织、培养细胞、福尔马林固定样品或血清中分离的非 polyA 尾 RNA，同时保持未扩增样品中发现的差异表达模式。
應用
SeqPlex™ RNA扩增试剂盒已用于扩增poly（A）筛选的RNA。
特點和優勢
- 少量总 RNA 随机引物技术可扩增来自所有来源（包括 FFPE 和 RIP）的片段化或完整 RNA。
- 半简并文库引物设计，可实现更完整的转录组覆盖和高效引发。
- 测序前无需进行 DNA的片段化。
- 在 8 小时或更短的时间内扩增 ds-cDNA。
- 兼容Pacific Bioscience以外的其它所有下一代测序平台。
其他說明
SEQR-500RXN 按需生产。请联系我们获取更多信息。
法律資訊
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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说明
价格
儲存類別代碼
11 - Combustible Solids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Cecilia Lindestam Arlehamn et al.
Journal of immunology (Baltimore, Md. : 1950), 193(6), 2931-2940 (2014-08-06)
In latent tuberculosis infection (LTBI) spread of the bacteria is contained by a persistent immune response, which includes CD4(+) T cells as important contributors. In this study we show that TB-specific CD4(+) T cells have a characteristic chemokine expression signature
实验方案
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
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