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一般說明
GenomePlex® 单细胞全基因组扩增试剂盒采用基于基因组 DNA 随机片段化的专有技术，并将产生的小片段转换为两侧有通用引物结合位点的 PCR 可扩增文库分子。 WGA 采用通用寡核苷酸引物进行文库分子 PCR 扩增。该试剂盒旨在扩增单个细胞的基因组。这种快速直接的方法实现了百万倍的扩增，从单个细胞制备微克级基因组 DNA。传统单细胞全基因组扩增方法得率不足，且存在明显偏差。该试剂盒包含细胞裂解和全基因组扩增所需的全部试剂。可以通过荧光激活细胞分选 (FACS)、激光捕获显微切割 (LCM)、稀释或任何其它适用方法分离单个细胞。
應用
GenomePlex® 单细胞全基因组扩增试剂盒已用于：
- 扩增分离的 DNA
- 全基因组扩增（WGA）
- 扩增显微切割的 DNA
- 从移性性去势抵抗性前列腺癌细胞中扩增循环肿瘤细胞基因组 DNA
- 通过单细胞低深度全基因组测序 （SLWGS）方法检测拷贝数变异 （CNV）
- 凝胶电泳
- qPCR
- 比较基因组杂交（CGH）微阵列
- 短串联重复序列（STR）分析
- 单核苷酸多态性（SNP）分析
特點和優勢
- 4 小时内高产量和准确的DNA 扩增
- 扩增任何来源的 DNA，例如癌细胞、上皮细胞、淋巴细胞、成纤维细胞羊膜细胞、聚碳酸酯固定细胞和植物细胞
- 完整表达整个基因组，等位基因偏差最小
- WGA DNA 聚合酶适用于下游应用，包括凝胶电泳、qPCR、比较基因组杂交（CGH）微阵列、短串联重复序列（STR）分析和单核苷酸多态性（SNP）分析、TaqMan ®分析和微卫星分析
其他說明
此试剂盒中提供的通用引物序列被认为是专有的。
法律資訊
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护： 5,789,224、5,618,711、6,127,155 和美国境外的权利要求，对应于过期的美国专利号 5,079,352。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
相關產品
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Nathan R Treff et al.
Molecular human reproduction, 16(8), 583-589 (2010-05-21)
Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two unique methods of aneuploidy screening of blastomeres within the same embryo has not been conducted and may indicate whether mosaicism has been overestimated
Nathan R Treff et al.
Fertility and sterility, 94(6), 2017-2021 (2010-03-02)
To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening. Prospective, randomized, and blinded study. Academic reproductive medicine center. Multiple euploid and aneuploid cell lines were obtained
Resolving tumor heterogeneity: genes involved in chordoma cell development identified by low-template analysis of morphologically distinct cells
El-Heliebi A, et al.
PLoS ONE, 9(2), e87663-e87663 (2014)
Heike Fiegler et al.
Nucleic acids research, 35(3), e15-e15 (2006-12-21)
Heterogeneity in the genome copy number of tissues is of particular importance in solid tumor biology. Furthermore, many clinical applications such as pre-implantation and non-invasive prenatal diagnosis would benefit from the ability to characterize individual single cells. As the amount
Jochen B Geigl et al.
Nucleic acids research, 37(15), e105-e105 (2009-06-23)
Clinical DNA is often available in limited quantities requiring whole-genome amplification for subsequent genome-wide assessment of copy-number variation (CNV) by array-CGH. In pre-implantation diagnosis and analysis of micrometastases, even merely single cells are available for analysis. However, procedures allowing high-resolution
商品
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
实验方案
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
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