推荐产品
一般說明
面向全基因组扩增（WGA）的 SeqPlex™ Enhanced DNA 扩增试剂盒专用于对极小量或降解/高度片段化的 DNA 进行下一代测序（NGS）。 染色质免疫沉淀（ChIP）或福尔马林固定的石蜡包埋组织样品（FFPE）的DNA含量往往无法满足成功制备下一代测序文库的需求。通过 SeqPlex 试剂盒可对这类 DNA 以及其他用于 NGS 的小量/高度片段化 DNA 样品进行预扩增。 该试剂盒是 WGA 产品线的延伸，旨在集成到 Illumina®、SOLiD™ 或 454 测序工作流程中。
應用
SeqPlex™ DNA 扩增试剂盒已用于全基因组扩增。
特點和優勢
- 采用随机引物技术扩增片段化DNA，如ChIP或FFPE
- 有助于对低至100 pg的ChIP DNA进行测序
- 采用增强型引物，可获得完整的基因组覆盖、最低的序列偏差、引物清除以及适用于二代测序的扩增子大小
- 可兼容用于二代测序的Illumina®、SOLiD™或454文库制备
其他說明
SEQXE-500RXN 是按需生产。获取更多信息，请发邮件至techserv@sial.com 联系技术服务。
法律資訊
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书
- Library Preparation Buffer
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA
Optimizing sparse sequencing of single cells for highly multiplex copy number profiling.
Baslan T, et al.
Genome Research, 25(5), 714-724 (2015)
SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.
Mian S A, et al.
Nature Communications, 6, 10004-10004 (2015)
实验方案
The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
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