推荐产品
等級
for molecular biology
for molecular biology
品質等級
無菌
0.2 μm filtered
產品線
BioReagent
形狀
solution
濃度
1 M
雜質
DNase, RNase, NICKase and protease, none detected
pH值
8.0
應用
agriculture
SMILES 字串
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI 密鑰
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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一般說明
Trizma®盐酸溶液是一种有用的生物缓冲剂。
應用
Trizma®盐酸溶液可用于以下研究：
- 作为大鼠成纤维细胞2-D电泳的缓冲液。
- 作为高分子量植物 DNA（50,000 碱基对或更长）的快速分离缓冲液。
- 生物素的选择性免疫沉淀标记的DNA与抗生素IgG和葡萄球菌 sp。
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。
对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
其他說明
用 pH 可调的生物技术性能认证的 Trizma 碱在 18 兆欧水中制备并经过 0.2μm 过滤器过滤。
法律資訊
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
其他客户在看
Lisa W von Friesen et al.
Marine pollution bulletin, 142, 129-134 (2019-06-25)
Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming.
P R Langer et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(11), 6633-6637 (1981-11-01)
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and
M G Murray et al.
Nucleic acids research, 8(19), 4321-4325 (1980-10-10)
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA
Emilie M F Kallenbach et al.
The Science of the total environment, 786, 147455-147455 (2021-05-09)
Chitinaceous organisms have been found to ingest microplastic; however, a standardised, validated, and time- and cost-efficient method for dissolving these organisms without affecting microplastic particles is still required. This study tested four protocols for dissolving organisms with a chitin exoskeleton:
F Gharahdaghi et al.
Electrophoresis, 20(3), 601-605 (1999-04-27)
Mass spectrometry is a powerful technique for the identification of proteins at nanogram quantities. However, some degree of sample preparation prior to mass spectrometry is required, and silver-stained protein gel samples are most problematic. Here we report our strategy to
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