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Merck

T2663

Sigma-Aldrich

Trizma® 盐酸盐 溶液

1 M, BioReagent, for molecular biology

别名:

Tris 盐酸盐溶液

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About This Item

MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology
for molecular biology

品質等級

無菌

0.2 μm filtered

產品線

BioReagent

形狀

solution

濃度

1 M

雜質

DNase, RNase, NICKase and protease, none detected

pH值

7.35-7.45

SMILES 字串

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI 密鑰

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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一般說明

Trizma ®盐酸盐溶液是一种有用的生物缓冲液。

應用

所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液,温度每降低 1°C,pH 增加约 0.03 个单位,浓度每稀释 10 倍,pH 降低 0.03-0.05 个单位。
对于精确应用,使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
采用 Trizma ®盐酸盐与 Trizma-base 制备 pH 7.35 的缓冲液。它被用于制备氚核浸提液。可用于从 嗜热脂肪芽孢杆菌枯草芽孢杆菌中分离的蛋白酶活性分析。可用作从肠球菌分离株和大肠埃希菌中分离的基因组 DNA 的限制性片段分析的缓冲液

其他說明

用 pH 可调的生物技术性能认证的 Trizma 碱在 18 兆欧水中制备并经过 0.2μm 过滤器过滤。

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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M Fujii et al.
Journal of bacteriology, 154(2), 831-837 (1983-05-01)
The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell
B E Murray et al.
Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
M Kasahara et al.
The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)
El-Refaie Kenawy et al.
Journal of controlled release : official journal of the Controlled Release Society, 81(1-2), 57-64 (2002-05-07)
Electrospun fiber mats are explored as drug delivery vehicles using tetracycline hydrochloride as a model drug. The mats were made either from poly(lactic acid) (PLA), poly(ethylene-co-vinyl acetate) (PEVA), or from a 50:50 blend of the two. The fibers were electrospun
Diego Piacentini et al.
Environment international, 132, 105094-105094 (2019-09-05)
Over the last years, various acellular assays have been used for the evaluation of the oxidative potential (OP) of particular matter (PM) to predict PM capacity to generate reactive oxygen (ROS) and nitrogen (RNS) species in biological systems. However, relationships

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