1.52028
Chromolith® NH2 (monolithic) HPLC Columns
L × I.D. 100 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® Performance NH2 100-4.6 HPLC column
About This Item
Recommended Products
product name
Chromolith® NH2, L × I.D. 100 mm x 4.6 mm HPLC column
material
PEEK column
Quality Level
agency
suitable for USP L8
product line
Chromolith®
feature
endcapped: no
packaging
1 ea of
extent of labeling
5% Carbon loading
parameter
200 bar pressure
45 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
10 cm × 4.6 mm
matrix
monolithic silica
matrix active group
NH2 bonding phase
pore size
13 nm mesoporosity
2 μm macroporosity
operating pH range
2-7.5
separation technique
hydrophilic interaction (HILIC)
normal phase
reversed phase
storage temp.
no temp limit
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General description
Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Analysis Note
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.3
Legal Information
Certificates of Analysis (COA)
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Articles
A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
Protocols
Separation of Xylose; Fructose; Glucose; Saccharose
Separation of Ribose, United States Pharmacopeia (USP) Reference Standard; Xylitol, analytical standard; Mannose, United States Pharmacopeia (USP) Reference Standard
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