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  • Determination of testosterone esters and estradiol esters in bovine and porcine blood serum.

Determination of testosterone esters and estradiol esters in bovine and porcine blood serum.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2016-12-06)
Martina Rejtharová, Libor Rejthar, Katarína Čačková
ABSTRACT

Monitoring of steroid esters in blood serum is desirable in order to detect the possible illegal use of natural hormones as growth promoters. A method for the determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate in bovine and porcine blood serum was developed. The procedure consists of protein precipitation and removal of phospholipids using a HybridSPE®-Phospholipid column followed by clean-up on a hydrophilic modified styrene polymer Supel

MATERIALS
Product Number
Brand
Product Description

Supelco
HybridSPE®-Phospholipid, Cartridge, bed wt. 500 mg, volume 6 mL, pk of 30