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  • Quantitation of human haptoglobin: comparative ELISA studies using adsorption and capture methods.

Quantitation of human haptoglobin: comparative ELISA studies using adsorption and capture methods.

Journal of immunological methods (1999-06-12)
E Yee, J E Brown
PMID10365789
ABSTRACT

Three ELISA methods for the quantitation of haptoglobin (Hp) in plasma and albumin are described: a polystyrene direct adsorption method and capture methods with antibody and hemoglobin. Hp aggregates generated by 60 degrees C heating showed as much as a hundred-fold higher response by polystyrene adsorption compared to the two capture methods, while unheated Hp showed comparable responses by the three methods.