Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig.

Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig.

Nature communications (2016-10-08)

Jianzhao Liu, Yuanxiang Zhu, Guan-Zheng Luo, Xinxia Wang, Yanan Yue, Xiaona Wang, Xin Zong, Kai Chen, Hang Yin, Ye Fu, Dali Han, Yizhen Wang, Dahua Chen, Chuan He

PMID27713410

ABSTRACT

DNA N6-methyldeoxyadenosine (6mA) is a well-known prokaryotic DNA modification that has been shown to exist and play epigenetic roles in eukaryotic DNA. Here we report that 6mA accumulates up to ∼0.1-0.2% of total deoxyadenosine during early embryogenesis of vertebrates, but diminishes to the background level with the progression of the embryo development. During this process a large fraction of 6mAs locate in repetitive regions of the genome.

MATERIALS

Product Number

Brand

Product Description

S1876

Sigma-Aldrich

D-Sorbitol, ≥98% (GC)

P8136

Sigma-Aldrich

Poly(vinyl alcohol), 87-90% hydrolyzed, average mol wt 30,000-70,000

P5655

Sigma-Aldrich

Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

S5011

Sigma-Aldrich

Sodium phosphate monobasic, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0% (titration)