- Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3.
Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3.
Chemical communications (Cambridge, England) (2005-05-19)
Dieter F Münzer, Peter Meinhold, Matthew W Peters, Sabine Feichtenhofer, Herfried Griengl, Frances H Arnold, Anton Glieder, Anna de Raadt
PMID15900339
ABSTRACT
Substrate engineered, achiral carboxylic acid derivative was biohydroxylated with various mutants of cytochrome P450 BM-3 to give two out of the four possible diastereoisomers in high de and ee. The BM-3 mutants exhibit up to 9200 total turnovers for hydroxylation of the engineered substrate, which without the protecting group is not transformed by this enzyme.
MATERIALS