- High-Throughput Production of Platelet-Like Particles.
High-Throughput Production of Platelet-Like Particles.
Methods in molecular biology (Clifton, N.J.) (2020-12-20)
Kylie M Persson, Pauline V Kneller, Mark W Livingston, Lucas M Bush, Tara L Deans
PMID33340367
ABSTRACT
The in vitro production of platelets could provide a life-saving intervention for patients that would otherwise require donor-derived platelets. Producing large numbers of platelets in vitro from their progenitor cells, megakaryocytes, remains remarkably difficult and inefficient. Here, a human megakaryoblast leukemia cell line (MEG-01) was used to assess the maturation of megakaryocytes and to develop a new methodology for producing high numbers of platelet-like particles from mature MEG-01 cells in vitro.
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Phorbol-12-myristate-13-acetate, Phorbol-12-myristate-13-acetate, CAS 16561-29-8, is the most common phorbol ester. Activates PKC at nanomolar concentrations.