K08670356
Kromasil® C18 HPLC Column
5 μm particle size, pore size 100 Å, L × I.D. 150 mm × 4.6 mm
Synonym(s):
Kromasil C18
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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agency
suitable for USP L1
product line
Kromasil®
manufacturer/tradename
Kromasil® KR100-5-C18-4.6X150
technique(s)
HPLC: suitable
L × I.D.
150 mm × 4.6 mm
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
100 Å
application(s)
food and beverages
separation technique
reversed phase
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General description
Reverse phase (RP) HPLC columns are majorly advantageous in ensuring high precision data, they are widely available commercially and are easy to use and less time-consuming. Two main approaches one should consider before choosing a RP column are, firstly characterising physiochemical parameters of the packing material such as 1% carbon load, surface area, pore size, particle size and pH. These are some of the important parameters important to control the quality of the column. Second approach is based upon the chromatographic and statistical evaluation of the retention behaviour for a large number of compounds. Kromasil C18 columns are basically RP columns made up of silica-based monomeric type reversed-phase packing material.
Legal Information
Kromasil is a registered trademark of EKA Chemicals AB
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Chromatographic classification of commercially available reverse-phase HPLC columns.
Cruz E
Chromatographia, 44 (3-4), 151-161 (1997)
M Kele et al.
Journal of chromatography. A, 855(2), 423-453 (1999-10-16)
The reproducibility of the retention data and the band profiles was investigated with Kromasil C18 columns (silica-based monomeric type reversed-phase packing material). High precision data were obtained and statistically compared among five columns from the same batch (column-to-column reproducibility) and
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