G1006621

GC Analysis of Terpenes in Cannabis on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber

suitable for GC/MS, suitable for solid phase microextraction (SPME)

Properties

• technique(s): GC/MS: suitable; solid phase microextraction (SPME): suitable
• test parameters: sample/matrix: 0.5 g dried cannabis in 10 mL headspace vial; SPME fiber: Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U); extraction: 20 min, headspace, 40 °C; desorption process: 3 min, 270 °C; sample preparation: 30 min equilibration at 40 °C prior to extraction; sample preparation: fiber postbake after extraction, 3 min at 270 °C; column: Equity-1, 60 m x 0.25 mm I.D., 0.25 μm (28047-U); oven: 60 °C (2 min), 5 °C/min to 275 °C (5 min); inj. temp.: 270 °C; detector: MSD; MSD interface: 300 °C; carrier gas: helium, 1 mL/min constant flow; liner: 0.75 mm ID, SPME
• application(s): food and beverages; vitamins, nutraceuticals, and natural products

Description

Analysis Note

Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. Cannabis sativa (cannabis) contains more than 100 different terpenes and terpenoids, including mono, sesqui, di and tri, as well as other misc. compounds of terpenoid orgin. Terpene profile does not necessarily indicate geographic orgin of a cannabis sample, however it can be used in forensic applications to determine the common source of different samples. In addition, different cannabis strains have been developed which have distinct aromas and flavors, a result of the differing amounts of specific terpenes present. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in dried cannabis. Sample was obtained courtesy of Dr. Hari H. Singh, Program Director at the Chemistry & Physiological Systems Research Branch of the National Institute on Drug Abuse at the National Institute of Health. The exact strain of the sample was not known. Chromatographic separation was performed on an Equity-1 capillary GC column, and identification was done using retention indices and spectral library match.

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Analyte

Hexanal
Hexene-1-ol
2-Heptanone
a-Thujene
a-Pinene + unknown
Camphene
6-Methyl-5-hepten-2-one
ß-Pinene
ß-Myrcene
Δ-3-Carene
a-Terpinene
Cymene
d-Limonene
γ-Terpinene
trans-Sabinene hydrate
cis-Linalool oxide
Linalool
d-Fenchyl alcohol
trans-Pinocarveol
Borneol L
1,8-Methandien-4-ol
p-Cymen-8-ol
Terpinene-4-ol
a-Terpineol
Piperitenone
Piperitenone oxide
a-Ylangene
a-Copaene
γ-Caryophyllene
a-Santalene
Caryophyllene
trans-a-Bergamotene + unknown
a-Guaiene
trans-ß-Farnesene
Humulene
Alloaromadendrene
a-Curcumene
ß-Selinene
a-Selinene
ß-Bisobolene
a-Bulnesene
Selina-3,7(11)-diene
Caryophyllene oxide
Humulene oxide
Caryophylla-3,8(13)-dien-5-ol A
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