G006146
HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3
application for HPLC
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technique(s)
HPLC: suitable
test parameters
column: Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)
mobile phase: (A) 10 mM sodium phosphate, pH 6.0; (B) A + 1 M NaCl
gradient: 0 to 100% B in 15 min
flow rate: 0.5 mL/min
detector: UV, 280 nm
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Legal Information
Proteomix is a registered trademark of Sepax Technologies
Analyte
Description
Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Cytochrome c from chicken heart, ≥95% based on Mol. Wt. 12,222 basis
Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid
Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein
Analytical column
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