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Key Documents

G005942

HPLC Analysis of mAb on Antibodix® WCX-NP5, 25 cm Columns

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Antibodix WCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777294)
mobile phase: (A) 10 mM sodium phosphate buffer, pH 7.5; (B) A + 100 mM NaCl
gradient: 15 to 55% B in 30 min
flow rate: 0.8 mL/min
column temp.: ambient
detector: UV, 214 nm
injection: 10 μL
sample: monoclonal antibody

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

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Antibodix is a registered trademark of Sepax Technologies

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