G005932
HPLC Analysis of a Peptide Drug on Antibodix® WCX-NP10, Gradient Optimization to Separate Impurity
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)
mobile phase: [A] 3 mM potassium phosphate, pH 5.0; [B] 20 mM potassium phosphate, pH 7.5
gradient: (A) 0% - 50% B (20 min); (B) 0% - 20% B (20 min), 20% B after 20 min
flow rate: 1 mL/min
column temp.: ambient
detector:
injection: 5 μL
sample: 2 mg/mL, proprietary peptide drug, pI 7-8
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
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