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G005679

HPLC Analysis of Bile Acids on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U)
mobile phase: [A] 10 mM ammonium acetate, pH 4 (adjusted with acetic acid); [B] acetonitrile; (80:20, A:B)
gradient: 20 to 100% B in 10 min
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ESI(-) in SIR mode
injection: 5 μL
sample: 10 μg/mL

suitability

application for HPLC

application(s)

clinical

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Dehydrocholic acid
    Cholic acid
    Chenodeoxycholic acid
    Deoxycholic acid
    Lithocholic acid

Analytical column

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