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G005674

HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl, Methanol Mobile Phase

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)
mobile phase: [A] water; [B] methanol; (35:65, A:B)
flow rate: 1.4 mL/min
pressure: 3600 psi (248 bar)
column temp.: 35 °C
detector: UV, 250 nm
injection: 2 μL
sample: 0.1 g/L in 20% methanol

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Analysis Note

The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Clonazepam
    Oxazepam
    Alprazolam
    Desmethyldiazepam
    Diazepam

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