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G005530

HPLC Analysis of Bisphenol A in Water on Ascentis® Express C18 after SPE using Supelclean™ ENVI-18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

SPE tube/cartridge: Supelclean ENVI-18, 500 mg/6 mL glass SPE tube (54331-U)
condition: 1 mL 1% formic acid in acetonitrile, then 1 mL water
sample: 5 mL spiked water blank, concentration of 0.2 μg/mL
elution: 2 mL 1% formic acid in acetonitrile
eluate post-treatment: 1 mL aliquot of eluent, and evaporate/reconstitute to 0.5 mL in acetonitrile
column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] acetonitrile; (60:40, A:B)


flow rate: 0.4 mL/min
pressure: 3270 psi (256 bar)
column temp.: 35 °C
detector: UV, 230 nm
injection: 1 μL

suitability

application for HPLC

application(s)

environmental

Analysis Note

Bisphenol A is a contaminant that can leach into aqueous solutions from polycarbonate plastics or metal cans; it may impact human development and reproduction. This application demonstrates the extraction and analysis of bisphenol A from water.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Bisphenol A, ≥99%

Analytical column

Product No.
Description
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SPE tube or plate

Product No.
Description
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Standard

Product No.
Description
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239658

Bisphenol A, ≥99%

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