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Key Documents

G005410

HPLC Analysis of Steroids on Ascentis® Express F5 with Methanolic Mobile Phase

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water [B] methanol; (40:60, A:B)
flow rate: 0.2 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 0.2 mL/min
sample: 50 mg/L in 75:25, water:methanol

suitability

application for HPLC

application(s)

clinical

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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