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G005374

HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

mobile phase: [A] 7 mM ammonium formate; [B] methanol; (25:75, A:B)
flow rate: 0.4 mL/min
column temp.: 40 °C
detector: UV, 250 nm

ESI(+), TIC m/z 100 - 1000
injection: 1 μL
sample: 300 mg/L in 25:75, water:methanol
column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
    25-Hydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
    25-Hydroxyvitamin D2 solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

Analytical column

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Standard

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mobile phase component

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