G004912

GC Analysis of Volatiles in Multifloral Honey on SUPELCOWAX 10 after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber

suitable for GC, application for SPME

Properties

• technique(s): gas chromatography (GC): suitable; solid phase microextraction (SPME): suitable
• test parameters: sample/matrix: 5 g multifloral honey + 5 mL bidistilled water in a 20 mL vial, allowed to equilibrate at 50 °C for 15 min with magnetic stirring; SPME fiber: 2 cm 50/30 μm divinylbenzene/carboxen on polydimethylsiloxane (57348-U); extraction: headspace, 50 °C for 40 min with magnetic stirring; desorption process: 250 °C for 2 min; column: SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 μm (24079); oven: 35 °C (8 min), 6 °C/min to 60 °C, 4 °C/min to 160 °C, 20 °C/min to 200 °C (1 min); inj. temp.: 250 °C; MSD interface: 230 °C; scan range: 35-300 m/z; carrier gas: helium, 1 mL/min; liner: 0.75 mm I.D., SPME; sample: (Honey)
• suitability: application for SPME
• application(s): agriculture; food and beverages

Description

Analysis Note

In this application, headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.

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Analyte

Nonanal
Furfural
Decanal
Benzaldehyde
Phenylacetaldehyde
Phenylethyl Alcohol
Nonanoic acid