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G004704

HPLC Analysis of Chlorthalidone Enantiomers on Astec® CYCLOBOND I 2000 RSP

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CYCLOBOND I 2000 RSP, 25 cm x 4.6 mm I.D., 5 μm particles (20324AST)
mobile phase: [A] 1% triethylamine acetate, pH 4.1; [B] acetonitrile; (95:5, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 254 nm
injection: 5 μL
sample: 2 mg/mL in methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Chlorthalidone, analytical standard

Analytical column

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