G004575
HPLC Analysis of FMOC-Isoleucine Enantiomers on Astec® CHIROBIOTIC® T
application for HPLC
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technique(s)
HPLC: suitable
test parameters
column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] 20 mM ammonium acetate, pH 4.1; [B] methanol; (60:40, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 260 nm
injection: 3 μL
sample: 1 mg/mL in methanol
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
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