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Key Documents

G004405

HPLC Analysis of Methylphenidate (Ritalin) Enantiomers on Astec® CHIROBIOTIC® T2

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T2, 25 cm x 4.6 mm I.D., 5 μm particles (16024AST)
mobile phase: 15 mM ammonium formate in methanol
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 220 nm
injection: 5 μL
sample: methylphenidate, 1 mg/mL in mobile phase

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Methylphenidate hydrochloride

Analytical column

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Description
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