G004366
HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid
application for SPE, application for LC-MS
About This Item
technique(s)
LC/MS: suitable
solid phase extraction (SPE): suitable
test parameters
SPE well plate: HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)
sample preparation: SPE (Solid Phase Extraction)
column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
sample/matrix: Rat plasma spiked with verapamil and metabolites at 10 ng/mL
mobile phase: [A] water; [B] acetonitrile, pH 2.55 adjust with formic acid (30:70, A:B)
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: TOF/MS
injection: 1 μL
sample addition: 100 μL spiked rat plasma followed by 300 μL 1% formic acid in acetonitrile
elution: apply vacuum
suitability
application for LC-MS
application for SPE
application(s)
clinical
Legal Information
Analytical column
SPE tube or plate
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