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G003764

HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18

application for SPE, application for HPLC

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About This Item

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: acidified horse serum spiked with indapamide at 10 mg/mL
SPE tube/cartridge: Discovery DSC-18, 50 mg/1 mL (52601-U)
condition: 1 mL methanol; 1 mL 10 mM potassium phosphate, pH 3
sample addition: 1 mL
washing: 10 mM potassium phosphate, pH 3
elution: 1 mL 60% methanol in DI water.
column: Discovery C18, 15 cm x 4.6 mm I.D., 5 μm particles (504955)
mobile phase: [A] 10 mM KH2PO4, pH 3 (adjusted with H3PO4): [B] acetonitrile; (60:40, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 234 nm
injection: 10 μL

suitability

application for HPLC
application for SPE

application(s)

forensics and toxicology

Application

Sample Pre-treatment:
Neat horse serum was acidified with 10 mL 6 M HCl/mL serum. Internal standard (I.S.) blank was prepared by spiking acidified horse serum with indapamide at 10 mg/mL. Sample A = 10 mg/mL furosemide in I.S. blank. Sample B = 5 mg/mL furosemide in I.S. blank. Sample C = 0.5 mg/mL furosemide in I.S. blank. Sample D = 0.1 mg/mL furosemide in I.S. blank. Sample E = 0.05 mg/mL furosemide in I.S. blank.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analytical column

SPE tube or plate


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