G002449
HPLC Analysis of Serotonin Metabolites on Ascentis® C18
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 10 mM formic acid/acetate (pH 3.0): [B] methanol; (80:20, A:B)
flow rate: 0.7 mL/min
column temp.: 35 °C
detector: ESI (+); overlay of extracted ion chromatograms of individual (M+H)+ species
injection: 10 μL
sample: 25 mg/L in 90:10, 10 mM formic acid/acetate (pH 3.0):methanol
suitability
application for HPLC
application(s)
clinical
Legal Information
Analytical column
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