820022
TSKgel® BioAssist® Chelate HPLC Column
phase iminodiacetic acid, L × I.D. 5 cm × 7.8 mm, 10 μm particle size, PEEK hardware
Synonym(s):
TSKgel® Chelate-5PW HPLC Column and Guardgel Kit
About This Item
Recommended Products
material
PEEK hardware
product line
TSKgel®
manufacturer/tradename
Tosoh 20022
extent of labeling
20 μmol/mL resin ligand density
parameter
≤20% organic solvent
≤3 M salt concentration
≤45 °C temperature
1.2 mL/min flow rate
10 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
5 cm × 7.8 mm
matrix
polymer particle platform
matrix active group
iminodiacetic acid phase
particle size
10 μm
pore size
1000 Å
operating pH
2-12
separation technique
affinity
General description
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
Legal Information
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Sorry, we don't have COAs for this product available online at this time.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service