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80023449

TSKgel® UP-SW3000 (2 µm) HPLC Columns

L x I.D. 15 cm × 4.6 mm, diol phase

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About This Item

UNSPSC Code:
41115700

product name

TSKgel® UP-SW3000 HPLC Column, phase diol, L × I.D. 15 cm × 4.6 mm, 2 μm particle size

material

stainless steel

agency

suitable for USP L20
suitable for USP L59

product line

TSKgel®

manufacturer/tradename

Tosoh 0023449

parameter

≤0.5 M salt concentration
0-30% aqueous soluble organic solvents
0.35 mL/min flow rate
10-30 °C temp. range
340 bar pressure

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

matrix

silica particle platform

matrix active group

diol phase

particle size

2 μm

pore size

250 Å

pH range

2.5-7.5

separation technique

size exclusion (SEC)

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Application

TSKgel® UP-SW3000 HPLC Column may be used for the separation of:
  • Free payloads and related species from antibody drug conjugates (ADCs) and their aggregates, during their determination by using 1-dimensional (1D) gradient size exclusion chromatography (SEC).
  • Protein biopharmaceuticals in aqueous solutions, during their analysis by SEC.

Other Notes

Related Catagories: Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel Gel Filtration Columns for Proteins and Other Biopolymers, TSKgel Gel Filtration Columns to Analyze Purity and Aggregates, TSKgel Size Exclusion Columns

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Comprehensive study on the effects of sodium and potassium additives in size exclusion chromatographic separations of protein biopharmaceuticals.
Goyon A, et al.
Journal of Pharmaceutical and Biomedical Analysis, 144, 242-251 (2017)
Extending the limits of size exclusion chromatography: Simultaneous separation of free payloads and related species from antibody drug conjugates and their aggregates.
Goyon A, et al.
Journal of Chromatography A, 1539, 19-29 (2018)

Articles

Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.

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