581400-U
Ascentis® C8 (3 µm) HPLC Columns
L × I.D. 5 cm × 2.1 mm, HPLC Column
Synonym(s):
C8 Reversed-Phase HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
Ascentis® C8 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 2.1 mm
material
stainless steel column
Quality Level
agency
suitable for USP L7
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
15% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
5 cm × 2.1 mm
surface area
450 m2/g
surface coverage
4.0 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle
silica gel high purity, spherical
matrix active group
C8 (octyl) phase
particle size
3 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.
The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.
Application
- Analysis of 27 beta-Blockers and Metabolites in Milk Powder by High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap High-Resolution Mass Spectrometry.: This study utilizes the Ascentis® C8 HPLC Column to analyze beta-blockers and their metabolites in milk powder, highlighting the column′s capacity for sensitive and precise quantification in complex matrices (Cheng et al., 2019).
- Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: A review of advancements in HPLC technology, including the use of Ascentis® C8 columns, which enhance the speed and efficiency of chemical separations (Ali et al., 2012).
- Superficially porous particles columns for super fast HPLC separations.: Discusses the benefits of using Ascentis® C8 HPLC Columns for achieving faster and more efficient separations in HPLC, underlining their utility in high-throughput settings (Ali et al., 2012).
- Gradient RP-HPLC method for the determination of potential impurities in atazanavir sulfate.: Demonstrates the effectiveness of the Ascentis® C8 HPLC Column in a robust method for identifying impurities in pharmaceutical compounds, ensuring product safety and compliance (Chitturi et al., 2011).
- Development and validation of an HPLC-MS/MS method to determine clopidogrel in human plasma. Use of incurred samples to test back-conversion.: Illustrates the application of Ascentis® C8 HPLC Column in a validated method for the analysis of clopidogrel, improving therapeutic drug monitoring and patient care (Silvestro et al., 2010).
Features and Benefits
- Superior retention for hydrophobic molecules
- Excellent peak shape
- Low bleed LC-MS separations
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Mahmoud Hasan et al.
Journal of pharmaceutical and biomedical analysis, 100, 341-347 (2014-09-10)
Clodronate belongs to the class of bisphosphonates which are used for the treatment of bone disorders. Due to its high polarity it has a low and highly variable oral bioavailability which results in low plasma concentrations and requires sensitive bioanalytical
Ebenezer B Asafu-Adjaye et al.
Journal of pharmaceutical and biomedical analysis, 43(5), 1854-1859 (2007-02-16)
Gastrointestinal stability of venlafaxine was evaluated in vitro in simulated gastric (SGF) and intestinal (SIF) fluids using a stability indicating HPLC method. The method was validated using a 5 microm Ascentis C18 column (150 mm x 4.6 mm) and mobile
Luana Magalhães et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1008, 98-107 (2015-12-08)
Nucleoside triphosphate diphosphohydrolase (NTPDase) is an enzyme belonging to the apyrase family that participates in the hydrolysis of the nucleosides di- and triphosphate to the corresponding nucleoside monophosphate. This enzyme underlies the virulence of parasites such as Leishmania. Recently, an
Ugandar Reddy Inugala et al.
Journal of chromatographic science, 51(5), 453-459 (2012-10-13)
This paper describes the development of a rapid, novel, stability-indicating gradient reversed-phase high-performance liquid chromatographic method and associated system suitability parameters for the analysis of naproxcinod in the presence of its related substances and degradents using a quality-by-design approach. All
Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
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Read our Biofiles article on Analysis of Isoprenoid Pathway Metabolites by LC-MS.
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