50487-U

Ascentis^® Express 90 Å Phenyl-Hexyl (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• Synonym(s): Core-shell (SPP) Fused Core Phenyl HPLC column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® Express Phenyl-Hexyl, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• agency: suitable for USP L11
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• parameter: 60 °C temp. range; 600 bar max. pressure (9000 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 90 m²/g
• impurities: <5 ppm metals
• matrix: Fused-Core particle platform; superficially porous particle
• matrix active group: phenylhexyl phase
• particle size: 5 μm
• pore size: 90 Å pore size
• operating pH: 2-9
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Application

• A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This study by Lhotská et al. explores the application of a fused-core column combined with on-line SPE-HPLC for rapid and automated analysis of ochratoxin A and citrinin in lager beers. The Ascentis^® Express Phenyl-Hexyl column demonstrates high efficiency and selectivity, significantly improving the throughput and sensitivity of the analysis (Lhotská et al., 2016).

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable