ZRB2442
Anti-AGTR1 Antibody, clone 1I12 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Anti-MXRA8 Antibody, clone 1F4 ZooMAb® Rabbit Monoclonal, AT1 receptor, AT1AR, AT1BR, Angiotensin II type-1 receptor, Type-1 angiotensin II receptor
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1I12, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 41.06 kDa
observed mol wt ~52 kDa
purified by
using Protein A
species reactivity
human, mouse
species reactivity (predicted by homology)
rat, canine, bovine
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... AGTR1(185)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1I12 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Type-1 angiotensin II receptor (AGTR1). It targets an epitope within 14 amino acids from the first extracellular domain in the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 14 amino acids from the first extracellular domain within the N-terminal region of human Type-1 angiotensin II receptor (AGTR1).
Application
Quality Control Testing
Evaluated by Western Blotting in Human liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AGTR1 in Human liver tissue lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound AGTR1 peptide with a KD of 4.0 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 Dilution of this antibody detected AGTR1 in HeLa, HepG2, and NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Human liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AGTR1 in Human liver tissue lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound AGTR1 peptide with a KD of 4.0 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 Dilution of this antibody detected AGTR1 in HeLa, HepG2, and NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Type-1 angiotensin II receptor (UniProt: P30556; also known as AT1AR, AT1BR, Angiotensin II type-1 receptor, AT1 receptor) is encoded by the AGTR1 (also known as AGTR1A, AGTR1B, AT2R1, AT2R1B) gene (Gene ID: 185) in human. AGTR1 is a multi-pass membrane glycoprotein of the G-protein coupled receptor family with seven transmembrane domains. It serves as a receptor for angiotensin II that acts as a key regulator of blood pressure and sodium retention by the kidney. The activated receptor couples to G- protein G(q) and thus activates phospholipase C and increases the cytosolic Ca2+ levels, which in turn triggers cellular responses such as stimulation of protein kinase C. Over-stimulation of ATGR1 leads to hypertension, cardiovascular hypertrophy, and fibrosis, whereas blocking its activity reduces blood pressure. Two subtypes of AGTR1 (AGTR1A and AGTR1B) have been described in human, mouse, and rat. They share substantial sequence homology and wide tissue distribution. AGTR1A predomina
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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