ZRB2222
Anti-FLT4 Antibody, clone 2F4 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Anti-TIM-3 Antibody, clone RMT3-23 ZooMAb® Rat Monoclonal, EC:2.7.10.1, Fms-like tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, Vascular endothelial growth factor receptor 3
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2F4, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 152.76 kDa
observed mol wt ~140 kDa
purified by
using Protein A
species reactivity
mouse, human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
ELISA: suitable
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... FLT4(2324)
Related Categories
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2F4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects FLT4/VEGFR 3. It targets an epitope within 26 amino acids from the N-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 26 amino acids from the extracellular domain within the N-terminal half of human FLT4/VEGFR3.
Application
Quality Control Testing
Evaluated by Western Blotting with His-tagged recombinant extracellular domain of human FLT4.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged recombinant extracellular domain of human FLT4/VEGFR3
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FLT4/VEGFR3 in bEnd.3 cells.
Affinity Binding Assay:: A representative lot of this antibody bound FLT4 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected His-tagged recombinant extracellular domain of human FLT4/VEGFR3.
Flow Cytometry Analysis: A representative lot detected FLT4/VEGFR3 in one million Human umbilical vein endothelial cells (HUVEC).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with His-tagged recombinant extracellular domain of human FLT4.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged recombinant extracellular domain of human FLT4/VEGFR3
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FLT4/VEGFR3 in bEnd.3 cells.
Affinity Binding Assay:: A representative lot of this antibody bound FLT4 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected His-tagged recombinant extracellular domain of human FLT4/VEGFR3.
Flow Cytometry Analysis: A representative lot detected FLT4/VEGFR3 in one million Human umbilical vein endothelial cells (HUVEC).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Vascular endothelial growth factor receptor 3 (UniProt: P35916; also known as EC:2.7.10.1, VEGFR-3, Fms-like tyrosine kinase 4, FLT-4, Tyrosine-protein kinase receptor FLT4) is encoded by the FLT4 (also known as VEGFR3) gene (Gene ID: 2324) in human. FLT4 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-775), a transmembrane domain (aa 776-796), and cytoplasmic domain (aa 797-1363). It is expressed in endothelial cells and is also detected in fetal spleen, lung, and brain. In adults, it is detected in liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. FLT4 has tyrosine-protein kinase activity and acts as a receptor for VEGFC and VEGFD. It plays a role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Its secreted fo
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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