ZRB2102

Anti-hnRNP A1 Antibody, clone 1I21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Heterogeneous nuclear ribonucleoprotein A1;Nuclear ribonucleoprotein particle A1 protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1I21, recombinant monoclonal
• description: 1I21 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 38.75 kDa; observed mol wt ~36 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• species reactivity (predicted by homology): feline, rat, canine, monkey, porcine, bovine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_112420
• UniProt accession no.: P09651
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... HNRNPA1(3178)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1I21 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1). It targets an epitope within 13 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hnRNP A1 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected hnRNP A1 in NIH3T3 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound hnRNP A1 with a KD of 5.1 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected hnRNP A1 in A431 and HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Heterogeneous nuclear ribonucleoprotein A1 (UniProt: P09651; also known as Nuclear ribonucleoprotein particle A1 protein) is encoded by the HNRNPA1 gene (Gene ID: 3178) in human. hnRNAP A1 is a member of the hnRNP family of conserved proteins that is involved in RNA transcription, pre-mRNA splicing, mRNA transport, protein translation, microRNA processing, telomere maintenance, and the regulation of transcription factor activity. It is ubiquitously expressed, however, due to post-translational modifications it can vary in its molecular function. It is mainly localized in cytoplasmic mRNP granules containing untranslated mRNAs and shuttles continuously between the nucleus and the cytoplasm along with mRNA. Its N-terminal region contains two RNA recognition motifs (aa 14-97 and 105-184) and the C-terminal region has the prion-like domain (PrLD) that mediates cellular compartmentalization, protein-protein interaction, and RNA-binding. The RRMs are composed of four β-sheets adjoined by two -helices (β1 1β2β3 2β4).The β-sheet surfaces primarily interact with RNA via amino acid stretches containing predominantly aromatic residues. hnRNP A1 is considered a key component of the response to cellular stresses and also binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1. Three isoforms of hnRNP A1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Clarke, JP., et al. (2021). Front. Mol. Biosci. 8; 659610; Li, ML., et al. (2019). PLoS One. 14(9); e0221048).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1