ZRB1917

Anti-NSE/Enolase-2 Antibody, clone 2J18-H1 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Gamma-enolase;EC:4.2.1.11;2-phospho-D-glycerate hydrolyase;Neural enolase;Neuron-specific enolase
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2J18-H1, recombinant monoclonal
• description: 2J18-H1 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 47.27 kDa; observed mol wt ~48 kDa
• purified by: using Protein A
• species reactivity: rat, mouse, human
• species reactivity (predicted by homology): monkey, canine, bovine, feline, porcine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminus
• Protein ID accession no.: NP_001966
• UniProt accession no.: P09104
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ENO2(2026)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2J18-H1 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Neuron-specific enolase (NSE/Enolase-2). It targets an epitope within 19 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Neuron-specific enolase (NSE/Enolase-2).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected NSE/Enolase-2 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected NSE/Enolase-2 in lysates from C6 cells, Rat brain and Mouse tissue, and SHSY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound NSE/Enolase-2 peptide with a KD of 4.5 x 10-9 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NSE/Enolase-2 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Gamma-enolase (UniProt: P09104; also known as EC:4.2.1.11, 2-phospho-D-glycerate hydrolyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE) is encoded by the ENO2 gene (Gene ID: 2026) in human. In vertebrates three isozymes of enolase have been described that are expressed by different genes. They are known as enolase , which is ubiquitous; enolase β is a muscle-specific form, and enolase is the neuron-specific form (NSE). NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. NSE exhibits neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It is expressed as - and as -dimer, is a late event in neural differentiation. Hence, it can serve as a useful index of neural maturation. Its levels in cord blood are useful in in confirming the diagnosis of neuroblastoma in newborns. It can also be used as a quantitative measures of brain damage following ischemic stroke, intracerebral hemorrhage, seizures etc. Increased NSE serum levels are also observed in various tumors. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Isgro, MA., et al. (2015). Adv. Exp. Med. Biol. 867:125-43).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable