ZRB1662
Anti-p-RPA2-Ser4/8 Antibody, clone 2F23 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34
About This Item
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2F23, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 29.25 kDa
observed mol wt ~35 and 38 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer4/8)
Gene Information
human ... RPA2(6118)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from HeLa cells treated with Etoposide.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected RPA2 phosphorylated on serine 4 and 8 in lysate from HeLa cells, overnight serum starved and treated with Etoposide ( 20 g/mL; 3 h)
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Phospho-RPA2-Ser4/8 in human lung cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Phospho-RPA32-Ser4/8 peptide with a KD of 2.7 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HeLa cells treated with Etoposide.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected RPA2 phosphorylated on serine 4 and 8 in lysate from HeLa cells, overnight serum starved and treated with Etoposide ( 20 g/mL; 3 h).
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
Not finding the right product?
Try our Product Selector Tool.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service