ZRB1612
Anti-Haptoglobin Antibody, clone 2D22 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Zonulin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2D22, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 45.21 kDa
observed mol wt ~40 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2D22 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects human Haptoglobin.
Immunogen
His-tagged full-length recombinant human Haptoglobin.
Application
Quality Control Testing
Evaluated by Western Blotting in Human liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Haptoglobin in Human liver tissue lysate.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Haptoglobin in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Haptoglobin with a KD of 8.9 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Haptoglobin in Human liver tissue lysate.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Haptoglobin in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Haptoglobin with a KD of 8.9 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Haptoglobin (UniProt: P00738; also known as Zonulin) is encoded by the HP gene (Gene ID: 3240) in human. Haptoglobin is a hemoglobin-binding acute-phase glycoprotein that is synthesized in the liver and is secreted in plasma. Although it belongs to the peptidase S1 family, it does not have any enzymatic activity. It is a tetramer of disulfide-linked two alpha and two beta chains and displays anti-inflammatory and antioxidative properties. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that can be further cleaved into an alpha (19-160) and a beta chain (aa 162-406). Haptoglobin complexes with free hemoglobin, resulting from erythrocyte hemolysis, and allows hepatic recycling of heme iron and prevents kidney damage. Hemoglobin/haptoglobin complex is rapidly cleared by the macrophage CD163 scavenger receptor expressed on Kupfer cells through an endocytic lysosomal degradation pathway. Its CD163 interacting region is localized to amino acids 318-323. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Schaer, DJ., et al. (2014). Front. Physiol. 5; 415).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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