ZRB1508

Anti-SOD1 Antibody, clone 1G13 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): EC:1.15.1.1, Superoxide dismutase 1, Superoxide dismutase [Cu-Zn]
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1G13, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 15.94 kDa; observed mol wt ~16 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_000445
• UniProt accession no.: P00441
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... SOD1(6647)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G13 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Superoxide Dismutase 1 (SOD1).

Immunogen

His-tagged, full-length (without the initiator methionine), recombinant human Superoxide Dismutase 1 (SOD1).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SOD1 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected SOD1in HepG2 cell lysate.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SOD1 in Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SOD1 in human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human SOD1 with a KD of 9.2 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Superoxide dismutase [Cu-Zn] (UniProt: P00441; also known as EC:1.15.1.1, Superoxide dismutase 1, SOD1) is encoded by the SOD1 gene (Gene ID: 6647) in human. Superoxide dismutases are metalloproteins that dismutate the superoxide radical (O2•) to hydrogen peroxide (H2O2) and molecular oxygen.  Three types of SOD isozymes have been identified in eukaryotic cells. Cu/Zn superoxide dismutase (SOD1) is predominantly located in the cytosolic fractions; Mn superoxide dismutase (SOD2) is located in the mitochondria, and the extracellular superoxide dismutase (SOD3) is localized in the extracellular space. SOD1 is a homodimeric enzyme that contributes to about 90% of the cellular SOD activity. It has a β-barrel structure that is stabilized by a Cys57-Cys146 disulfide bridge and a zinc ion in the active site. The two subunits are held together by strong hydrophobic forces, which makes it one of the most compact and stable protein. Each subunit also contains a copper ion that undergoes alternative oxidation-reduction cycle in the course of the dismutation of superoxide to oxygen and H2O2. Although it is predominantly expressed in the cytoplasm, it is also found in the nucleus and mitochondria. In addition to its antioxidant properties, it is also implicated in signal transduction, and both its wild-type and mutant forms are prone to misfolding. A vast number of mutations have been recorded in SOD1, some of these mutations cause amyotrophic lateral sclerosis (ALS), a neurodegenerative disorder predominantly affecting upper and lower motor neurons. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Berdynski, M., et al. (2022). Sci. Rep. 12; Article 103; Forsberg, K., et al. (2019). Neurol. Neurosurg. Psychiatry 90(8);  861-869).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable