ZRB1429

Anti-p27 Kip1 Antibody, clone 1B10 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B10, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 22.07 kDa; observed mol wt ~30 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Designing Safer Chemicals; Design for Energy Efficiency; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminus
• Protein ID accession no.: NP_004055
• UniProt accession no.: P46527
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B10 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects p27 Kip1. It targets an epitope within 18 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Cyclin-dependent kinase inhibitor p27 (p27 Kip1).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected p27 Kip1 in Jurkat cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p27 Kip1 in HeLa cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound in p27 Kip1 with a KD of 2.5 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p27 Kip1 in HeLa cells and HUVEC.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected p27 Kip1 in human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-p27 Kip1, clone 1B10 ZooMAb^®, Cat. No. ZRB1429, is a recombinant Rabbit monoclonal antibody that detects p27 Kip1 and is tested for use in in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Cyclin-dependent kinase inhibitor 1B (UniProt: P46527; also known as Cyclin-dependent kinase inhibitor p27, p27Kip1) is encoded by the CDKN1B (also known as KIP1) gene (Gene ID: 1027) in human. p27Kip1 is a member of the universal cyclin-dependent kinase inhibitor family that serves as an important regulator of cell cycle progression. It inhibits the kinase activity of CDK2 bound to cyclin A. Its expression is regulated by cell contact inhibition and by specific growth factors. p27Kip1 is shown to be essential for the establishment of a G1 check point arrest after DNA damage. Phosphorylation of p27Kip1 by ATM kinase leads to its stabilization following DNA double strand breaks. In addition to its role as a cyclin-dependent kinase inhibitor p27Kip1 also acts as a tumor suppressor, regulator of drug resistance in solid tumors, and promoter of apoptosis. In quiescent cells it localizes in nucleus and cytoplasm and its levels are high that decline rapidly after stimulation with mitogens. Phosphorylation on serine 10 is the major site of phosphorylation in resting cells that takes place at the G0-G1 phase and leads to protein stability and facilitates nuclear export. Akt or Rsk- mediated phosphorylation on threonine 197 increases its interaction with 14-3-3 protein and allows it to translocates to the cytoplasm and promotes cell cycle progression. When phosphorylated on tyrosine 88 and 80, p27Kip1 translocates to the nucleus. Its nuclear localization signal is localized in amino acids 153-169. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lloyd, RV et al., (1999). Am. J. Pathol. 154(2): 313-323; Cassimere, EK et al., (2016). PLoS One. 11(9); e0162806).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable